The husband and wife gave an emotional performance together at the concert in Central Park.
It is just weeks since Beyonce and Jay Z's 'On The Run' tour ended in Paris, but the couple were back on stage together again last night (27th September) at the Global Citizens Concert in New York.
Beyonce made a surprise appearance during Jay Z's performance in Central Park, joining her husband for 'Holy Grail' and 'Forever Young'.
"New York City, make some noise for Beyonce," the rapper said, as he walked out alongside his wife. More than 60,000 people enjoyed the couple's passionate performance together, which included Beyonce singing the chorus of 'Holy Grail'.
The concert's aim is to end extreme poverty by 2030 and also saw No Doubt and Alicia Keys perform.
(Warning: this video contains strong language that some people may find offensive.)